Sentence examples for expanded detached from inspiring English sources

To facilitate the close tip approach, the rough and wrinkled NE was expanded, detached from the nucleoplasm, and smoothened by swelling the nucleus in a hypotonic buffer solution containing 5.5 g/L PVP.

Fully expanded leaves were detached and scanned.

Per plant 5 6 fully expanded leaves were detached and, if necessary, the midrib excised with a scissor (Additional file 1).

Alternatively, fully expanded leaves were detached and subjected to drought stress treatment by placing on lab blench or on water-saturated filter papers in Petri dishes as controls in detached leaf assays.

Twenty of the second fully expanded leaves were detached from atop stolons and placed on a glass slide in immersion oil to maintain the original state of the stomates.

The new model predicts the evolution of the cell number for different sub-populations: adult cells consisting of budding mother cells and adult cells waiting in G1 phase; daughter cells consisting of buds attached to mother cells in G2 or M phase, expanding newborn cells detached from the mother cells and daughter cells from the former generation.

iPSCs colonies were manually detached and expanded as individual clones in the same media.

The expanded cell populations were detached from flasks using 1 × Trypsin/EDTA solution, in preparation for either passaging or xenograft injection.

Culture expanded ASC samples were detached from plastic support with trypsin/EDTA (Euroclone) counted and aliquoted in FACS analysis polypropylene tubes (0.5 1 × 10/tube) (Falcon).

Of paramount importance, the debate on expanding access cannot be detached from the need to ensure quality.

Adherent cells were allowed to expand to near confluence, detached using 500 µg mL−1 trypsin and 200 µg mL−1 EDTA4Na (Invitrogen) and reseeded at a density of approximately 500 cells per cm2.