During the Younger Dryas age, woolly mammoths briefly expanded into north-east Europe, whereafter the mainland populations became extinct.
Colonies were isolated using a pipette tip and briefly expanded in adherent cultures before isolation of genomic DNA using the DNAeasy kit (Qiagen).
Following this first boost, Ad5-specific CD8+ T-cell responses returned to pre-vaccination levels in baseline Ad5 seropositive subjects, and were only briefly expanded again at weeks 30 and 42 following the 2nd boost at week 26 (p<0.04).
Fibroblasts explanted from donors of different ages, then briefly expanded in culture, also showed a moderate reduction in HuR levels as the age of the donor increased [ 49].
After SCA1+CPC transduction of the indicated lentiviral vectors (Supplementary Figure S2a), positively transduced cells were sorted, briefly expanded, and total RNA was isolated using the mirVana kit (Ambion, Madrid, Spain).
Briefly, expanded OC-derived cells and sorted OC cells were released by trypsin treatment, counted and resuspended in 15 ml polypropylene conical tubes at a density of 2 × 10 10, and short spun down at 500 g.
Although the extent of rainforests briefly expanded again during the early Pliocene due to a temporary return to warm and wet conditions, by the end of the Pliocene open woodlands, sclerophyllous forests and grasslands dominated the landscape of eastern Australia [ 3, 6, 8, 10].
"What kind of message are we sending if our kids don't feel safe, don't feel safe simply walking to the store to get candy and a drink?" Fulton addressed the panel only briefly, but expanded on her views in written testimony presented for the senators' consideration.
Briefly, five expanded leaves (the 4 8th true leaves) per plant were manually inoculated with purified TMV-Cg (10 µg/mL in 10 mM sodium phosphate buffer, pH 7.5).
