Exact(17)
Mesenchymal stem/stromal cells (MSCs) were isolated, expanded and characterized as described before95.
hBM-MSCs were expanded and characterized by immunofluorescence and FACS analysis.
Mesenchymal stem cells were isolated, expanded, and characterized following standard protocols.
The hBMSCs were harvested, expanded and characterized with respect to their differentiation potential and population distribution.
The selected clones were subsequently expanded and characterized as previously described47 to confirm expression of pluripotency proteins TRA-1-60 aNANOGNOG, and SNP analysis was conducted to confirm normal karyotype and for cell line tracking, using Illumina OmniExpress24 array (700,000 markers).
TILs from 40 melanoma tissue specimens were expanded and characterized.
Similar(43)
As a first step, we performed preclinical work to expand and characterize melanoma TILs.
First, the bone marrow (BM) obtained using needle aspiration was used for to isolate, expand and characterize the BM-MSCs.
We report here a method that enabled us to recover, expand, and characterize regulatory T-cell clones specific for a dominant autoantigen in a patient with autoimmune disease.
After being isolated, HUCPVCs were expanded and then characterized to use during in vitro and in vivo procedures.
The cells are isolated, expanded and extensively characterized (see URL link for characteristics of the cells, including phenotypic analysis, proliferation rate, expansion data, colony forming units, and trilineage differentiation into mineralizing cells, adipocytes and chondrocytes: http://www.som.tulane.edu/gene_therapy/distribute_docs/Spec_Sheet.pdf).edu/gene_therapy/distribute_docs/Spec_Sheet.pdf
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