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In order to significantly expand the transcript catalog of watermelon and gain more insights into the molecular basis of watermelon fruit development, we performed large-scale transcriptome sequencing of watermelon fruits using the Roch/454 massively parallel pyrosequencing technology.
In order to significantly expand the transcript catalog of A. mongolicus, identify candidate genes involved in drought tolerance, and develop more SSR markers, we performed large-scale transcriptome sequencing of A. mongolicus root using Roche/454 next-generation sequencing technology.
Thus it's very important to expand the transcript catalog of watermelon in order to facilitate gene discovery, functional analysis, molecular breeding, and comparative genomics analysis of watermelon and its closely-related species.
This observation supports the idea that alternative splicing events increased during evolution from the unicellular ancestor to the multicellular alga Volvox in order to expand the transcript diversity and, as a consequence, the proteomic diversity.
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These various evolutionarily conserved transcripts expand the transcriptional range of activity of Mef2c proteins.
We undertook to expand the melon transcript catalog in the framework of the International Cucurbit Genome Initiative, which was established in 2005, being one of its major objectives to sequence approximately 100,000 ESTs from different melon genotypes and tissues [ 28].
Ultra-deep sequencing methods, in particular, could help to expand the oak transcript catalog for studies of the genomic mechanisms underlying plastic responses and evolutionary adaptation to environmental change.
In this study, using 454 pyrosequencing technology, we analyzed the overall transcriptional profile of olive (cv. Picual) fruit pericarp at full ripening to significantly expand the olive transcript catalog.
In order to better understand the genetic and molecular basis of these changes and significantly expand the watermelon transcript catalog, we have selected four critical stages of watermelon fruit development and used Roche/454 next-generation sequencing technology to generate a large expressed sequence tag (EST) dataset and a comprehensive transcriptome profile for watermelon fruit flesh tissues.
As a result of this analysis, we were able to annotate thousands of signatures matching predicted genes, quantify the expression level of these genes in the developing berries, compare the expression profiles derived from ESTs and MPSS signature frequencies, and expand the coverage of known transcripts in an important grapevine organ at a specific developmental stage.
The MPSS data presented here not only achieved a higher level of saturation than previous EST based analyses, but in doing so, expand the known set of transcripts of grape berries during the unique stage in development that immediately precedes the onset of ripening.
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