Sentence examples for exogenous standard from inspiring English sources

An internal exogenous standard (cDNA synthesis with no reverse transcriptase) was also run separately for each cDNA mixture.

Normalization using exogenous standard gives information about mRNA isolation and RT reaction efficiency and removes errors resulting from changes in mRNA level of endogenous standard.

Prior to cDNA synthesis, an exogenous standard of A. thaliana (RCA) mRNA (Stratagene, L Jolla, CA, USA) was added (0.1 ng) to each CM, CD, LM, and LD total RNA (2 μg) sample for normalization of succeeding gene expression data.

Next, we performed qRT-PCR with normalization to an exogenous standard (cyc3GFP), which was added to each sample as in vitro synthesized and polyadenylated cycGfp mRNA, prior to mRNA isolation from the samples.

All data were standardized using exogenous standards.

To determine the absolute quantity of each phospholipid, exogenous standards of PC (17:0/17:0), PE (17:0/17:0), and PG (17:0/17:0) that are absent in R. palustris TIE-1 were added as internal standards for LC-MS analyses.

The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard.

An exogenous glutamate standard (5 nmol) was added at the end of each experiment.

General information of 8 exogenous lipid standards used for lipidomics analyses were summarized in the Supporting Information Table S1.

The spiked concentrations of 8 exogenous lipid standards used in lipidomics analyses and normalization strategies used for LC MS lipidomics data analyses were summarized in the Supporting Information Table S2.

The response to exogenous ALA under standard conditions (the ALA phenotype) is characteristic for each cell type.