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Biomedical imaging uses exogenous probe molecules that often contain metal ions, metalloids or halogens (Zeglis & Lewis 2011; Blower 2015).
Therefore, it is of great needs to develop exogenous probe with long absorption wavelength and high sensitivity for photoacoustic imaging of orthotopic brain glioma.
Moreover, the possibility of detecting collagen as a harmonophore via TCSPC-SHG, without the need for an exogenous probe, may be advantageous to measure ex vivo cartilage degeneration (density and homogeneity of the collagen matrix).
Therefore, a sphingolipid-targeted, exogenous probe for live imaging studies would be a useful tool in studying diseases whose pathogenesis is glycosphingolipid-dependent.
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These results demonstrate a new technique for in situ mapping of single cell viability without any exogenous probes that is capable of providing continuous monitoring of construct health.
In this work, we demonstrate that optical coherence microscopy (OCM) can be used to assess cell viability without the addition of exogenous probes in three-dimensional (3D) tissue scaffolds maintained under standard culture conditions.
The introduction of exogenous probes into living cells inevitably interferes with endogenous signaling activity and thus often requires verification of the functionality and disrupting-effect of the probe.
Both endogenous molecules and exogenous probes can be molecular imaging agents" [ 45].
A series of exogenous probes of known concentration are used, allowing observed variation to be attributed specifically to the assay.
Exogenous probes with no sequence homology to human RNA sequences are included as positive and negative assay controls.
The method relies on the ability of oxygen to quench emissive triplet states of exogenous probes dissolved in biological fluids.
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