Exact(2)
Thus, [ PSI + ]Weak propagation is similarly sensitive to elevated temperature in exponentially growing cultures and in those that have recently exited stationary phase.
In a culture that recently exited stationary phase, the size of SDS-resistant Sup35 aggregates increased, as assessed by semi-denaturing detergent agarose gel electrophoresis (SDD-AGE) (Kryndushkin et al., 2003), following incubation at 40°C and a 2 hr recovery at 30°C (Newnam et al., 2011), consistent with an inhibition of fragmentation (Newnam et al., 2011).
Similar(58)
We created PGM null mutants in E. coli and discovered the ΔdPGM mutant grew slowly due to a delay in exiting stationary phase.
This growth lag was seen as a delay in exiting stationary phase in the ΔdPGM strain relative to ΔiPGM and MG1655.
This phenotype of ΔdPGM on solid medium confirms that observed in liquid culture, suggesting a general problem in exiting stationary phase in ΔdPGM cells.
We report that loss of dPGM leads to delayed growth both in liquid cultures and on solid medium, apparently due to a delay or defect in exiting stationary phase.
To allow sufficient time for recB strains, which grow slowly, to exit stationary phase, these were incubated in growth medium for an additional 30 min prior to thymine starvation (Materials and Methods).
Cells were grown to stationary phase in M9 minimal medium with 50 μg/ml thymine, 0.1% glucose, and 0.5% casamino acids (thy+ growth medium) then diluted 1 20 into fresh thy+ growth medium, and incubated at 37° for ∼1 hr 10 min (1 hr 40 min for strains containing a Δ recB allele) to allow them to exit stationary phase and enter early log phase.
As cells exit stationary phase, TAG hydrolysis supplies precursors for membrane lipid synthesis.
When the missing nutrient is added again and the cells exit stationary phase, these phenotypes are rapidly reversed, indicating that PKA activity in the cells increases to the high level typical for fermentatively growing cells (Thevelein & de Winde, 1999).
The regulated genes reflect the transition towards glucose metabolism, the resumption of growth and the release of stress, similar to cells exiting a stationary growth phase.
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