elegans genes to show an unexpected divergence in the expression profiles of these two nematodes in dauer and dauer exit samples.
We have chosen the same 12-hour time-point for the P. pacificus dauer exit samples after verifying that the post-dauer development follows similar kinetics in both species.
(2) At the 12 hour time-point (the stage of dauer-exit samples), no discernible morphological differences are found between the recovered dauers from both species.
Our experimental setup contrasted gene expression in the dauer stage (time point 0 hour) with the dauer-exit samples (time point 12 hour) via a common reference sample (mixed stage) for each species.
Second, at the 12-hour time-point (the stage when we collect the dauer-exit samples), no discernible morphological differences are found between the recovered dauers from both species.
Furthermore, other clusters from later dauer-exit time points in the same study show a significant overlap with genes called over-expressed in our dauer-exit samples (Table 6, rows 2 to 4, column "cel_exit"), thus indicating good agreement between the two data-sets.
Interestingly, the clusters corresponding to "early" and "climbing" genes in C. elegans (Table 6, rows 2 and 3) also show a significant overlap with genes over-expressed in our P. pacificus dauer-exit samples (column "ppa_exit" in Table 6), pointing towards a conservation of a part of the active transcriptome during dauer recovery in the two species.
Still, the exit poll sample size was especially small in Vermont, so we should watch carefully as actual results trickle in.
You can try to control for this problem by noting the basic demographic facts of non-respondents, but you can't possibly know someone's education or income level simply by looking at them. 3. A perfect exit poll sample may not be "perfect".
People exit the sample by death, moving abroad or refusal to interview.
