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Autograft and allograft have been used for this purpose but there exists disadvantages that cannot be overlooked, for instance donor site pain and morbidity, blood loss, infection, slow rate of incorporation and secondary fractures.
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Therefore, despite their great utility in many applications, there exist disadvantages to draft genome sequencing that need to be addressed by appropriate experimental design and deployment of sequencing resources to effectively answer the study questions posed.
Among them, bio-nanopore sequencing exists the disadvantages of DNA molecules' pause and reverse, which makes the current-time signal detected by this method misinterpreted [55].
But there also exists some disadvantages with respect to fluid delivery, such as bad symmetry, large system disturbance, low efficiency, and necessary consideration of the on-way flow resistance.
Though the LUT-based inverse halftoning method is easily implemented, there exists a disadvantage of this method, the constructed LUT information must be sent to the receiver.
This finding highlights that relative disadvantage exists within and across remote Aboriginal communities and measuring disadvantage should not be restricted to comparing only that between broad Indigenous and non-Indigenous population groups.
Although the above disadvantage exists, the lumped-masses model is simple and intuitive.
This disadvantage exists because these layouts are generally designed for a given product mix and demand volume in a fixed planning horizon.
In marked contrast to the findings from advanced democracies that incumbent office holders enjoy electoral advantages, this study finds that a substantial incumbency disadvantage exists in South Korean National Assembly (KNA) elections.
"This health disadvantage exists at all ages from birth to age 75 and that even advantaged Americans those who have health insurance, college educations, higher incomes, and healthy behaviors— appear to be sicker than their peers in other rich nations.
Disadvantage exists not only through socioeconomic status [ 98] and income inequalities [ 132] but also through economic stress and security, with metaflammation as a possible link [ 133].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com