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2) Requiring the use of a mutant transposon and hence existing transposition vectors must be mutated.
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Transition from a linear to circular economy is not straightforward and there are very few existing examples of the transposition of the EU's 'Circular Economy Action Plan' into national or regional policy.
Existing methods can still be applied when transposition is the dominant event.
It has been suggested that multiple mechanisms contribute to sex chromosome turnover, including a new master sex-determining gene arising on an autosome, the fusion of an existing Y chromosome with an autosome, and by transposition of an existing sex-determining gene to an autosome (Kitano and Peichel 2012).
Subsequent to this major organismal lineage split, the four existing SR genes underwent independent duplications, losses, and transpositions, leading to the current SR phylogeny of Drosophila and mosquitoes.
These interpretations suggest that the formation as well as further transposition of novel inverted repeat sequences are facilitated by existing transposable elements, and could contribute towards the evolution of new mobile elements.
Finally, we exploited an existing model for diversity-dependent speciation to test the hypothesis of transposition regulation.
Five ATon families contain elements existing at more than 10 identical copies, suggesting very recent or current transposition activity.
Messiaen continually evolved new composition techniques, always integrating them into his existing musical style; his final works still retain the use of modes of limited transposition.
The class II elements transpose via a DNA intermediate that is either excised from its existing location via double-stranded cleavage followed by subsequent insertion elsewhere or by a phenomenon involving transposition in which only a single strand is cleaved (e.g., rolling circle replication, [ Kapitonov and Jurka 2001]).
The automation procedure was completed using existing liquid handling infrastructure in a modular fashion, limiting custom-designed elements to a minimum to facilitate transposition.
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