Exact(6)
Sequences with greater than 80% identity to an existing repeat sequence were removed.
Since the k-mer and de novo methods do not rely on the existing repeat datasets, they are also suitable for identifying novel repeats in a genome.
Instead, we suggest that the strong GO term associations previously attributed to TNR tracts are indicative of opportunistic use of existing repeat sequences, a position supported by the cross-species nature of the associations observed above.
To characterize sequence repeats, we applied a combined similarity and de novo approach to improve upon our existing repeat library and to better define the components of the largest portion of the genome.
obs,) to scan these data, many of the clones with one different end sequence were found to be cases where the shared end sequence was in fact repetitive but not masked using existing repeat databases.
In addition to an existing repeat library, TREP complete annotation (http://wheat.pw.usda.gov/ITMI/Repeats/, 20 September 2013, date last accessed), a de novo repeat family constructed using RepeatModeler (http://www.repeatmasker.org/RepeatModeler.html, 20 September 2013, date last accessed), was used for repeat detection.
Similar(54)
GFP-DMPK 3'UTR(CTG)400 was constructed by cloning an uninterrupted tract of ∼400 CTG repeats at the site of the existing (CTG 5 repeat sequence in the GFP-DMPK 3'UTR(CTG)5 plasmid.
To control for pyrosequencing read errors in homopolymeric regions due to non-linear light response [12], any mismatch that was an extension/truncation of an existing homopolymeric repeat was removed from further analysis.
DNA sequence analysis demonstrates that the deletion created a novel, complete Alu repeat by homologous recombination between two existing Alu repeats that flanked the deletion.
We found that the co-existing repeat families in Zea and Coix comprised only 16 clusters.
Thus, C. lacryma-jobi and C. aquatica HG shared more co-existing repeat families with Sorghum than with Zea.
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