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The auto-radiography of the 35S short pulse labeled gel provides the information about protein newly expressed without interference from the former existed amount.
This contrast between PH-I and CBB measurements shows another merit of the 35S metabolic labeling, high signal/noise ratio, just reflecting the protein newly expressed within the 15 min and without the interference from the former existed amount.
Assuming the total protein amount at time point one was 100+1 (100 for the already accumulated proteins and 1 for the newly synthesized), at time point two is 100+5, then if judged from the already existed amount by CBB, the difference is (105−101)/101 = 104%, which would be considered as "no change".
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how do they exist?—amount to category mistakes, equivalent to asking the color of virtue.
The judge said the harm to the first folio, of which only 228 still exist, amounted to the "cultural vandalisation" of a "quintessentially English treasure".
The Supreme Court said that "to compel a homosexual person to pretend" that his sexuality does not exist amounted to denying "his fundamental right to be who he is".
A conspiracy between big business, the police and the security services, and the refusal to disclose information that everyone knows exists amounts to a good old-fashioned establishment cover-up.
Those who do not believe that there are any fictional entities (fictional antirealists, as we shall call them) will claim that the nonexistence datum has an ontological reading only: to say that fictional entities do not exist amounts to saying that in the overall domain of what there is there are no such things as fictional entities.
The surveys that do exist measure a tiny amount of actual forest, and so what might be verified is widely spaced.
For the %Volume data of existed proteins' total amount (sexisted), which is from the CBB staining image, we interpolate them with piecewise polynomial of the cubic spline, and then take its first order derivative, which approximately indicates the function of the variation speed (vexisted) of the total amount of existed protein (sexisted) with respect to time (t), during these six hours.
Since high-temperature was applied, possibility of formation of hydrothermal degradation products existed, therefore total amounts of degraded quercetin and formed 5-hydroxymethylfurfural were determined.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com