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In addition to protein arrays, other techniques exist for protein quantification, such as mass spectrometry (MS).
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Hence the question remains: can localization microscopy be used for protein quantification and extract reliable numbers of molecules that exist in specific subcellular compartments at a given time?
The remaining tissue fraction was used to extract live cells for protein quantification.
An aliquot was kept for protein quantification using the Lowry method.
Bias Correction and Background Correction was checked for protein quantification and normalization.
After centrifugation at 12,000 rpm for 5 min, supernatant containing acid-soluble proteins was transferred into fresh vial for protein quantification and western blot.
The most widely used technique for protein quantification is ELISA.
BCA kit purchased from Thermo Fisher Scientific was used for protein quantification.
For protein quantification in the whole-cell lysates, the protein-dye-binding method of Bradford [11] with commercially available reagents was used (Bio-Rad Laboratories, HerCAles, CA, USA).
Supernatants were used for protein quantification.
Aliquots of lysates were used for protein quantification by Bradford (Biorad), stored at −80°C.
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