However, significant challenges exist for generation of whole genome sequence data, largely because even the most sensitive sample preparation kit for the generation of sequencing libraries demands at least 1 ng genomic DNA; C. parvum oocysts each contain 40 fg DNA [ 5] thus requiring an estimated 2.5 × 10 highly-purified oocysts.
He said: "Entrenched poverty, for instance, has existed for generations.
But many town residents dislike redrawing boundaries that have existed for generations.
They've co-existed for generations with the buses, the park rangers like to say, and so many don't regard their approach as cause to run.
Faced with continuing financial pressures to cut, sell off or simply abandon swaths of local government services that have existed for generations, English local authorities inevitably clutch at any cash straws they can.
The backbone of the German economy is its Mittelstand – a core of small and medium-sized firms, many of which have existed for generations and have proved their durability and resilience.
Although organisations like these have existed for generations (in the early 1800s, the British and Foreign Anti-Slavery Society played a powerful part in abolishing slavery laws), the social and economic shifts of this decade have given them new life.
"The adoption of the O.E.C.D standards is a significant signal that bank secrecy as it has existed for generations is coming to an end," said Scott D. Michel, a tax lawyer at Caplin & Drysdale in Washington, D.C. Switzerland is not abandoning its banking secrecy laws, but rather adopting the definition of tax evasion used by the 30-nation Organization for Economic Cooperation and Development.
Those informal networks of support, which existed for generations, were the precursors to modern trade unions.
Interesting that witchhunting, just like voodoo and witchery, is something that has existed for generations.
