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The Y96L mutant, however, exhibits a marked decrease in kcat by >8-fold from 847 to 96 min–1.
To provide additional evidence that LuPDCT1 and LuPDCT2 encode functional proteins, their corresponding open reading frames were expressed in the seeds of the A. thaliana rod1 mutant, which exhibits a marked decrease in 18 2 and 18 3 PUFAs, along with a concomitant increase in 18 1 relative to WT plants [ 12, 33].
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Similarly, preadipocytes from IRS1 KO mice exhibit a marked decrease in differentiation and lipid accumulation [20], [23], [42].
Conversely, estrogen receptor beta (ESR2), G-protein coupled receptor 85 (SREB2) and the mesothelial fibroblast growth factor 9 (FGF9) exhibited a marked decrease of BRCA1 binding upon protein methylation inhibition.
As shown in Figure 6, rats injected with shamporter + nephrin siRNA exhibit a marked decrease in nephrin specific staining, compared with animals injected with control siRNA.
Our data (Figure 5) show that mitochondrial cytochrome c content was maintained with ACET treatment, whereas P2VEH rats exhibited a marked decrease in cytochrome c content.
The population of BAR-T cells infected with pSuper-p53RNAi exhibited a marked decrease in baseline p53 expression, with a selected clone showing complete elimination of baseline p53 expression (Figure 1A).
As demonstrated in Fig. 8B, cells transfected with active heparanase exhibited a marked decrease in expression of MMP-2 (5.8 fold), MMP-9 (6.5 fold) and MMP-14 (3 fold), a mirror image of the increased expression found in Hpse-KO mice.
We observed that after treating cells for 48 h with the SCD1 inhibitor, cancer cells exhibited a marked decrease in the content of both cyclin D1 and CDK6 (Fig. 1B).
At four days after eclosion these flies exhibited a marked decrease (60%) in their climbing ability becoming almost immobile by day 15, while the control classes were very active at this time (Figure 8C).
Comparison of 2D gels revealed that in the absence of inflammatory stimulus CF cells exhibit a marked decrease in the expression of TRX-1, PRDX-1 and 6, catalase and GST-pi, but a marked increase in SOD2 compared with normal matched cell pairs (Table 1).
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