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The majority of the down-regulated mRNAs exhibit seed homologies with the introduced miRNAs.
For example, 13% of conserved miRNAs exhibit seed shifting, and we describe arm-switching events in 11% of orthologous pairs.
Around 13% of conserved miRNAs between Drosophila and Tribolium exhibit seed shifting, and we describe arm-switching events in 11% of the orthologous pairs.
39, 46 As most target prediction algorithms rely on evolutionary conservation to distinguish authentic target sites from the multitude of heptamer segments that would exhibit seed complementarity by chance, 18 tackling ORFs for target site searches was somewhat challenging, where strong conservation has been imposed to preserve the codon usage.
Similar(56)
Candidate promoter sequences have also been identified from other fruits that exhibit seed-specific expression in heterologous species, indicating the presence of conserved cis-elements.
This may explain the fact that there are very few known endogenous targets on the 5'-UTR that exhibit seed-matches [ 29].
The integration of high-resolution QTL mapping with differential expression profiling detected six including one potential serine carboxypeptidase gene with ILP markers (linked tightly to the major seed weight QTLs) exhibiting seed-specific expression as well as pronounced up-regulation especially in seeds of high (ICC 4958) as compared to low (ICC 2263) seed weight mapping parental accessions.
One SNP (G/A -carrying CSN8 G/A -carrying, at CSN8W1.1 QTL interval, exhibited seed-specific expression (compared with leaves) as well as pronounced up-regenetiof (>7-folds) in high seed weightheseentat genotype (ICaqSW1.1) and homozygous mapping individuals during seed development (Fig. 4B).
Also, combining Rf6 with Rf5 (Rf1a) in most pre-existing BT-type restorers may be an effective way to breed new BT-type restorers that exhibit stable seed setting abilities.
Forty-one percent (136) of these exhibit a seed phenotype when disrupted by a loss-of-function mutation.
We demonstrated through reverse genetics that knockouts of that second locus (SALK_050034; SAIL_874_A12) did not exhibit a seed phenotype.
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