Interestingly, the 2.7 Mb deleted X minichromosome exhibits reduced stability when transferred from the hamster cell background into different mammalian cells [21], [22].
By contrast, in the absence of ongoing protein synthesis, all four truncation mutants exhibited reduced stability, with less than 20% of initial levels of mutant Hhat protein remaining after 24 hrs (Fig. 2).
The unmodified T·G mispair also exhibits reduced stability (50).
At the structural level, H2A.Z has been shown to enhance the stability of the nucleosome [ 15, 16] despite the fact that the H2A.Z-H2B dimexhibitsits a reduced stability compared to H2A-H2B dimers [ 16, 17].
Most unstable complexes (t1/2<1h) contained amino acid substitutions at position5, previously identified as an anchor residue for Db, although clearly there are exceptions since the lower affinity and reduced stability exhibited by peptide A3V are accounted for by the change at position3.
This translates to a reduced stability of coordination.
In addition, such precipitates may exhibit reduced long-term stability.
This result agrees with a previous report indicating that the D17N mutant exhibited reduced thermal stability compared to the wild-type enzyme (Arnórsdóttir et al. [2011]).
As a consequence of their flexible nature in the unbound state, unmodified short peptide sequences often exhibit reduced target affinity and low proteolytic stability as well as cell permeability, thus limiting their use as biologically active agents.
The exception to this relationship is that variants containing R384G without E375G exhibit reduced growth even when they possess adequate stability and activity.
Some of the type 1 VHL mutant proteins have also been shown to exhibit reduced binding to elongin C, which is important for pVHL stability [ 30, 53].
