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ABR and BCR have both GAP and GEF domains but only exhibit GAP activity in vivo.
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In contrast, the gradients intrinsic to compression sleeve design exhibit gaps in the middle range (1 5 N) of physiological strains and also inconsistencies along the length of the sleeve, resulting in less than optimal performance of these devices.
As expected, most of the 52 clones sequenced herein exhibit gaps and are non-active elements.
Chromosomal fragile sites are the regions of the genome which exhibit gaps or breaks on metaphase chromosomes under conditions of partial replication stress [ 42].
Fragile sites are defined as non-random chromosomal loci that exhibit gaps and breaks on metaphase chromosomes under conditions which partially inhibit DNA synthesis [ 6].
While CYK-4 GAP exhibits GAP activity towards both RhoA and CED-10/Rac1, CYK-4R459A GAP lacks detectable GAP activity towards either GTPase.
Similarly Legionella pneumophila virulence factor LepB exhibits GAP activity towards host cell Rab1 GTPase to disrupt proper membrane cycling and activation [43].
DLC2 contains a RhoGAP domain and exhibits GAP activity on RhoA and Cdc42 in vitro.
Furthermore, 37,719 (97.83%) unigene CDSs had no gaps at all and 610 (1.79%) exhibited gap lengths less than 10% of the total length.
GRAF1 exhibits GAP activity for the small G proteins RhoA and Cdc42 and has been shown to interact with the kinases FAK and PKNβ [5, 6, 14].
Then, the ratio of gap length to length of scaffold was analyzed; 81,058 (84.22%) scaffolds had no gap at all, 10,216 (10.61%) had gap lengths less than 10% and only 1,018 (1.06%) exhibited gap lengths ranging from 20 40% of the total length.
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