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Note that the difficulty of clan AA is not to obtain an exhaustive alignment but to exhaustively align all its possible sequence forms.
The loss of collinearity and orthologues through evolutionary time tends to render exhaustive alignment useful or practical only for closely related genomes.
We adopt a sequence alignment program, exonerate, which allows approximation to exhaustive alignment based on dynamic programming [ 19].
Kraken first identifies candidate regions for projection from the synteny graph and then performs an exhaustive alignment to return base-accurate lift-over annotations.
Exhaustive alignment and phylogeny approaches cannot be applied to data sets with > 10 sequences, but heuristic approaches have been developed that sacrifice the guarantee of optimality for the benefit of being able to run on much larger sequence data sets.
We have used the exhaustive alignment feature of ISAS (Imagenix, USA) to systematically generate uniqueome data for human (hg18 and hg19), mouse (mm9), worm (ce6) and fly (dm3) genomes in both color-space and nucleotide-space.
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The first exhaustive protein alignment benchmark [ 10] used the so called BAliBASE (Benchmark Alignment dataBASE) [ 11].
Briefly, iterative assembly uses an in silico restriction map of available genome sequence resources--contigs, scaffolds, pseudomolecules, etc.--as a reference for exhaustive pairwise alignment [ 25] of entire Rmap datasets.
Investigation of ambiguities in the assemblies was carried out using a set of scripts to carry out exhaustive local alignments.
Reads were mapped to the reference genome (Ka4C1) using Smalt v.0.74 with options –x (more exhaustive search for alignments) and –y = 0.8 (filters output alignments by a threshold in the number of exactly matching nucleotides).
Our algorithm could also find an exhaustive set of alignments up to a given score level by searching at that score level and reporting all results.
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