As part of validation, data collected on an IAEA inter-comparison exercise sample are presented under short and long counting durations with pump operating and switched off conditions respectively.
Additional file 3: Estimated number of true cases and reported cases for the verification exercise sample size calculations.
The protocol outlines the justification for using utility values in LMICs, selection of EQ-5D-3L and EORTC-8D health states, the TTO exercise, sample size calculation, sample selection and approach to face to face interview of participants.
This was in contrast to Allobaculum, Aggregatibacter and Suturella where both were more abundant in pre-exercise samples.
The first (rest) samples were collected around 6 a.m., and the second (post-exercise) samples were collected 8.30 a.m. from race horses and 5 p.m. from endurance horses.
Regarding pre-exercise samples, the proportion of sequences from the Bacteroides acidifaciens species was significantly more abundant in Obese rats than in Wistar and Hypertensive rats (p < 0.05).
Despite minimal variation in the relative abundance of Allobaculum between pre-exercise and post-exercise samples, this genus was enriched by exercise training (p < 0.05).
The lack of changes could be expected because post-exercise samples were collected approximately 2.5 hours after the rest samples, which was most likely too early to detect an increase in the SAA concentration.
It also indicated that microbiota from Wistar, Hypertensive and Obese rats were significantly altered by exercise training, where pre-exercise samples clustered significantly far from fecal samples collected after four weeks of exercise training.
Thus, Firmicutes were more abundant in post-exercise samples compared to pre-exercise in obese rats (Obese rats; 0.69 ± 0.03 vs. Exercised Obese rats; 0.78 ± 0.04, p < 0.05), while Bacteroidetes was shown to be reduced after training only in WR (Wistar rats; 0.23 ± 0.04 vs. Exercised Wistar rats; 0.17 ± 0.03, p < 0.05).
