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To measure the possible different temperatures inside the excreta heap one heap also had temperature loggers placed in the top and bottom.
There was no difference in the total die-off time between eggs in the tea bags inserted in the top and bottom of the excreta heap with 0% lime.
Figure 2 shows the temperature development in the top versus the middle of the excreta heap and the ambient temperature as measured every four hours during the 120-day duration of the experiment.
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The excreta heaps were then inoculated with helminth eggs (Ascaris suum) and their survival studied to assess the hygienic quality of the composted excreta.
Temperature increases in the excreta heaps due to microbiological processes were only seen to a limited extent and only within the first three weeks of storage.
Each bag contained approximately 20,000 A. suum eggs and a nylon fishing line was attached to allow for easy removal of the bags from the excreta heaps.
This showed a slower die-off rate for the excreta heaps with 0% lime and a more rapid initial egg die-off for the heaps with the highest pH.
This egg stock solution, which contained approximately 2.2 × 104 eggs/ml, was stored in the refrigerator at 4-5°C 4-5°C three days before being inserted into the excreta heaps.
When adjusted for counts of survived eggs in the control bags, a mean of 3% of the eggs placed in the five experimental excreta heaps were alive after 88 days (range 9 - 1%), 1% after 103 days (range 2 - <1%) and <1% after 117 days of storage.
To obtain different alkaline conditions, four heaps were added with 1%, 3%, 6% or 11% hydrated lime w/w (mass lime per mass wet excreta) and one heap without lime (control).
It was decided to carry out the experiment in heaps of excreta rather than directly in vaults as it was impractical to inoculate helminth eggs (see below) in the middle of a heap inside the vault in such a way that the individual bags with eggs could be removed for analyses without disturbing the remaining parasite egg bags.
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