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However, for genes with detectable small RNA but no TE, the gene expression levels are often very high, and small RNA sequencing identifies predominantly or exclusively sense-strand derived fragments with high proportions of 25-28 nt RNAs and little noticeable 5' nucleotide bias.
In the 23 and 35 DAP libraries, only 68.3% and 74.6% of the respective genes were matched exclusively by sense tags (Table 2).
Analysis of the gene coverage using reads mapping to unique locations of the B. taurus reference genome based exclusively on sense strand sequence information, showed that of the 25,669 annotated B. taurus genes, 15,422 genes (60.1%) had at least one sequence read count (i.e. one mapped read) in at least one library.
Interestingly, only 44, of the 188 398 reads containing SL sequences, were mapped in antisense orientation (related to the coding strand), suggesting that trans-splicing occurs almost exclusively in sense transcripts and that antisense transcripts (if produced at meaningful levels) should not be processed by the addition of mini-exon sequences.
Naqvi, Murtaza, and Aslam [45] define a smart PU emulation attacker as one that emulates PU activity exclusively during sensing times in the CR network.
In this case, the challenge lies almost exclusively in sensing accurately the primary activity, a topic that lies outside the scope of this paper (see, e.g.[11] and references therein).
Similarly we saw that mosquito piRNAs mapping to the various gypsy elements were almost exclusively anti-sense, consistent with a primary biogenesis by PIWI, though as yet we do not know if there is a functional equivalent of flamenco as a piRNA master locus in the mosquito, or if in the somatic follicle only PIWI is expressed.
These quartets occur either exclusively on the sense or anti-sense strand, or on both strands.
Sequence design occurs either exclusively on the sense or anti-sense strand, or on both strands depending on the SNP.
Moreover, small RNA of the highly expressed genes predominantly or exclusively matched the sense strand (i.e. the mRNA itself), while sense and antisense strands were roughly equally expressed from genes where mRNA was expressed at lower levels (Fig. 3c).
They depend exclusively on your sense of individuality, i.e., your "consciousness".
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