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Further, the exclusive miRNAs viz.
In addition, we observed a high induction of novel exclusive miRNAs candidates in the salt stress libraries, where we identified twice as many novel miRNA expressed under the stress condition than in the control samples (59 to 101).
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Figure 8 and S6 (Additional file 2) display the complementarity between the dataset-exclusive miRNAs and their (miRanda + MapMi -predicted PLA2 and SVMapMi -predictedoci listed in Figure 7.
Only seven PMRD-exclusive miRNA families mapped against small RNA cassava libraries; these were taken into account for pre-miRNA identification but no precursor could be identified in the cassava genome and were excluded from later analysis.
More significant is the fact that a C. s. simus newborn venom gland-exclusive miRNA and a venom gland miRNA uniquely found in adult snakes target, respectively, mRNAs encoding a PIII-SVMP and the B-subunit of crotoxin/Mojave toxin.
The binding of an miRNA to an mRNA is not exclusive, as a single miRNA can target hundreds of mRNAs, and a single mRNA can be affected by multiple miRNAs (Selbach et al, 2008).
Furthermore, most of the pre-miRNA candidates (93%) harbour a unique miRNA leading to an exclusive miRNA/pre-miRNA association (Additional file 3: Figure S3b).
However, in sensitive genotypes under drought stress the number of exclusive new miRNAs decreased weakly (27 to 22).
MiRanda predicted 10 miRNAs complementary of 3'-UTR loci of C. s. simus SVMP 454-transcripts (5 shared between newborn and adult; 5 newborn-exclusive) and 3 miRNAs from adult snakes targeting PLA2 mRNAs.
Previous studies have shown exclusive expression of some miRNAs in certain tissue types [ 47, 54].
The biotic stress library showed higher exclusive expression of new miRNAs (155 sequences).
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CEO of Professional Science Editing for Scientists @ prosciediting.com