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For the female patient, three iPS clones differing for CDKL5 allelic expression were used: clone#20 that exclusively expresses the wild type CDKL5 allele and clone#46 and clone#33 with exclusive expression of the mutated CDKL5 allele.
We have developed different AO cocktails and report a more effective combination of AOs that induced almost exclusive expression of the desired transcript missing both exons, first in vitro and then in vivo.
The finding of almost exclusive expression of the pre-piRNAs in pachytene spermatocytes was unexpected, as it is in contrast with the postmeiotic abundance of their mature forms [4].
Of interest, PMC42 cells, described as having stem cell-like properties [ 17], showed exclusive expression of the CD44S isoform.
The exclusive expression of the β-chain-containing FcεRI in murine cells is because all three chains have to be present for cell surface expression [ 10].
For C. eugenioides, the CaCc/ CaCe expression was extremely low, which validates that there is almost an exclusive expression of the CaCe isoform in this species.
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The virtual lack of Dp260 and Dp116 expression confirms earlier hypotheses on the exclusive expression of these isoforms in the retina and peripheral nerves, respectively, despite a recent report indicating that Dp260 may be expressed in brain as well44.
The specific functions of these transcripts involved in spermatogenesis, explain the exclusive expression of these transcripts in the testis.
The lack of strong upregulation of this group of genes in the IN5 sample, and the exclusive expression of this group in xylem from a tissue type gene expression dataset, illustrates its predominant role in xylem secondary wall biogenesis.
Genomic imprinting results from the epigenetic modification of a gene or gene region that leads to the mutually exclusive expression of either the maternal or the paternal allele.
The results showed a preferential expression of the mutant PRPS1 allele in the c.343A>G carrier of Family 2 (60% vs 40%) (Supplementary Figure 2A) and an almost-exclusive expression of the wild-type PRPS1 allele in the c.925G>T carrier of Family 3 (85% vs 15%) (Supplementary Figure 2B).
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