Sieve mesh sizes 40, 45, 50, 60, 70, and 80 (Fisher Scientific, Waltham, Massachusetts) with exclusion sizes of 425, 355, 300, 250, 212, and 180 μm, respectively, were used for each analysis.
Enhanced fluidity (EF) liquid mixtures are advantageous as mobile phases for the separation of moderate to polar compounds in liquid chromatography (reversed-phase, normal, size exclusion, size exclusion, and chiral separations).
Dynamic exclusion was used with the following parameters: exclusion size 500, repeat count 2, repeat duration 30 s, exclusion time 180 s, exclusion window ±6 ppm.
The standard dynamic exclusion list applied had a repeat count of 1, a repeat duration of 30 s, an exclusion size of 100, an exclusion duration of 180 s, and an exclusion mass width of 1.50.
The dynamic exclusion function for previously selected precursor ions applied the following parameters: repeat count of 2, repeat duration of 45 seconds, exclusion duration of 60 seconds, and exclusion size list of 150.
In the fully open state its apparent diameter is about 3 nm [22] and its solute exclusion size ≈ 1500 Da.
Dynamic exclusion was enabled with an exclusion size list of 500, exclusion duration of 60 s, and a repeat count of 1.
Proteins were concentrated by ultracentrifugation (21,000 g, 20 min, 4°C) using Vivaspin 500 columns (exclusion size 30 kDa, Sartorius AG, Göttingen, Germany).
This results indicates that the PVM possesses pores with an exclusion size of <10 kDa similar to most studied hemichannels [ 100].
The other method as aim of size exclusion nanotubes uses size exclusion chromatography and porous filters [37] as well as ultrasonically assisted microfiltration which purifies SWNTs from amorphous carbon and catalytic particles [38].
This correlation was statistically robust to the inclusion or exclusion of size.
