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Every cell inside the box was counted that had a visible nucleolus that did not touch the exclusion line.
If the BEB is intact, the molecules accumulate along the border of the eye forming a hemolymph exclusion line (Pinsonneault et al., 2011).
A counting box of 100 × 100 μm (with a buffer zone of 5 μm corresponding to the exclusion line on either surface was employed to exclude the cut surfaces) was centered on the marked intersections.
Similar(57)
The counting frame displayed inclusion and exclusion lines and only immunoreactive cell bodies falling within the counting frame with no contact with the exclusion lines were counted.
Only Islet-stained nuclei within the counting frame and no contact with exclusion lines were counted using a 40× objective.
Cells were only counted if they did not touch the exclusion lines and if they came into focus within the 18-µm thick optical dissector, with 2-µm guard zones.
Axons in the frame or at the (dashed) inclusion lines were counted provided that they do not in any way touch the (solid) exclusion lines or their infinite extensions.
The exclusion of line 2 was not intentional but was due to the late completion of F3 for that line.
According to the Cabinet Office note, poor performance under previous contracts is to be explicitly permitted as grounds for exclusion in line with the government's view that ineffective or poorly-performing suppliers should not be awarded public contracts.
The molecular weights of several poly(TBAm) samples determined by four independent analytical techniques, size exclusion chromatography/on-line light scattering detector (SEC/LSD), 1H NMR, 13C NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), were in agreement, close to the theoretical ones.
After exclusion of lines not common to the two data sets and markers with >20% missing data, the final data set consisted of 259 individuals scored for 847 SNPs and 89 SSRs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com