To exclude variations in the expression level of Ostm1 to be responsible for this inhomogeneous distribution, 24 single clones of the stably expressing cell line were analyzed.
Our method of colour measurement only includes the human-visible spectrum (approximately 400 700 nm) and excludes variation in the ultraviolet range, which is visible to the birds (e.g. Cuthill et al. 2000).
To exclude variations in signal intensities due to different positions within the cell cycle, only G1 cells were analyzed.
RNA stability controls were performed by northern blots to exclude variations in RNA amounts or stabilities that may account for the differences in translation efficiency.
These results show that the geomagnetic activity (described by Ap) is strongly linked to the solar cycle phase (solar activity is described by F10.7) and in this study, we were unable to exclude variations in foF2 related to geomagnetic activity.
Silhouette quality and information correctness: Our dataset has relatively high-quality silhouette images because we manually checked each silhouette not only to ensure image quality, but also to exclude variations in walking conditions to enable a pure investigation of gait-based age estimation.
One positive bat serum specimen was used as a reference to exclude variations in experimental procedures and signal intensities.
However, we cannot exclude variations in post-transcriptional regulation, which might still result in different levels of active proteins at the site of inflammation.
We can, however, not exclude variations in post-transcriptional modification, and we are unable to measure whether there are differences in Dicer protein level between patients and controls.
Discrimination of weak and strong positive staining was based on the intensity observed in different components within the same area of the tissue section to exclude variation in staining intensity caused by differences during the fixation procedure.
