Excluding the step of DNA extraction, the test results could be generated approximately within 1 h.
Extraction of total RNA from zebrafish embryos was performed using the mIRVANA miRNA isolation kit (Invitrogen) excluding the step for enrichment of miRNA.
Thus, the preparation of colon targeted drug delivery system of poorly soluble drug using liquisolid technique could exclude the step of preformulation study and the colon drug delivery system could be prepared directly in one step.
We adopted a similar approach used in other studies [ 30] and diverged from the methodology described by Levac and colleagues [ 31] by excluding the final step of a six step framework, stakeholder consultation, which was not relevant for this study.
Although this model excludes the early steps of metastasis, gastric tumours are known to metastasise to the peritoneal cavity, and the presence of gastric tumour cells in the peritoneal cavity is a well-described marker for identifying patients who are at increased risk of peritoneal recurrence (Burke et al, 1998; Hayes et al, 1999).
All steps excluding the incubation of DNA, EC buffer, Enhancer reagent and Effectene reagent were undertaken in 10% FBS-containing medium.
This resulted in a total analysis time (excluding the DNA extraction step) of less than 75 min.
B-lymphocyte differentiation is associated with the expression of a variety of cell surface markers, including CD19, CD20, C22, CD40, surface Ig, and BAFF-R present at the different maturation steps, excluding the end-stage of this B-cell lineage, plasma cells [ 6– 9].
The numerical results obtained from the proposed theory for simply-supported and clamped plates with stepped thickness, excluding the effect of additional mass, show good agreement with results obtained from the finite element method using FEM code NASTRAN.
In this case, the function associated with that protein subset is enucleated from the former FN, which should be viewed as excluding the enucleated function (step 5 of the algorithm pseudocode).
