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Approaches to reducing systematic errors by excluding problematic data, which often include fast-evolving or saturated sites, also have been suggested for plastid genome analyses [ 20, 41, 80, 110, 122].
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The ratio was well preserved through the initial amplification and after post-processing filtering to exclude problematic sequences and to trim error-prone ends.
We implemented an anatomically guided sampling strategy to selectively exclude problematic sampling points from image reconstruction, illustrated in Fig. 2.
Likewise, we excluded problematic plants that may be descended from a crop but could also be descended from a close wild relative of the crop.
All microarray scans were subjected to standard quality control measures, as detailed in the methods section, in order to exclude problematic samples.
Quality controls were applied to the data obtained from each OA community separately to exclude problematic samples and single nucleotide polymorphisms (SNPs).
Furthermore, we showed that excluding these problematic sites did not preclude measuring relative ASE for most exons, although the most rapidly evolving exons are expected to be preferentially eliminated.
Both studies used a Portuguese-language version of the SWBQ, and both concluded that a model which excluded three problematic items offered the best fit to the data.
Although the EAL writers in the present study have demonstrated their awareness of the SoP's general genre features without formal SoP writing instruction, we should keep in mind that the study's data selection process excluded most "problematic" SoPs.
To make our dataset more conservative, we further excluded potentially problematic orthologs such as those with short sequence lengths and those involved in horizontal gene transfer (HGT).
For this reason, we excluded the problematic tissues, leading to a subset of 26 non-gender-specific tissues and 5 gender-specific tissues.
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