Your English writing platform
Discover LudwigExact(1)
A near identical peak substructure was identified excluding fragmentation bias as the origin of the observed patterns and indicating that the observed substructure has a biological rather than technical origin (Fig. 2B), however effects of PCR amplification and/or variation in context-dependent sequencing efficiency cannot be excluded as factors that contribute to the observed patterns.
Similar(59)
By excluding the fragmentation step, full length transcripts containing the SOLiD P1 PCR primer at the 5′ end.
Acceptable metrics in all five measurements are required for glycan compositional confirmation and the four variables (excluding insource fragmentation) required for annotation (see the Methods section).
Already fragmented ions were excluded from fragmentation for 45 s.
The MS/MS experiments were carried out in auto MS/MS mode using a 4 Da window for precursor ion selection, an absolute threshold of 10,000, after 3 MS/MS spectra, the precursor ion were excluded from fragmentation for at least one minute.
Monoisotopic precursor selection was on, precursors with charge state 1 were excluded from fragmentation.
Parent ions with a charge state from z=1 and unassigned charge states were excluded for fragmentation.
The MS/MS scan was acquired at a resolution of 17 500 with a dynamic m/ z range, an AGC target value of 100 000, and a maximal IT of 200 ms. Fragmented precursor ions were excluded from fragmentation for 30 s.
Full scans were obtained between 350 and 1600 m/z followed by 12 MS/MS scans on the top multiple charged precursor ions using higher-energy collisional dissociation (HCD) at a normalized collision energy of 30%, which were then excluded from fragmentation for 45 s.
TOF/TOF tandem MS fragmentation spectra were acquired for each ion, averaging 4000 laser shots per fragmentation spectrum (excluding trypsin autolytic peptides and other known background ions).
The washing steps of the thylakoid membranes with sodium pyrophosphate, sucrose and one of the wash steps with fragmentation buffer were excluded before Yeda press fragmentation.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com