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An additional molecular study was performed in one case, excluding alterations of SIL-TAL1, HOX11L2, HOX11, CALM-AF3, MLL and CALM-AF5 genes.
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By contrast, we excluded alterations in hair follicle morphogenesis or cycling or in the differentiation of hair follicle keratinocytes.
To exclude alterations in the expression of DISC proteins due to clonal selection in sh-VDAC1 cells, the integrity of key components of the DISC was examined.
Our findings do not address effects in peripheral systems nor do they exclude alterations in the trafficking of cells and virus at the choroid plexus.
Although the reduced expression of fibronectin may account for this finding, we cannot exclude alterations in other extracellular matrix components, or alterations in the levels of other cell surface integrins as being involved in this response.
However, in some studies the endpoints were clearly not comparable, including one [ 42] where the endpoint was any type of oral lesion and one [ 47] where the definition specifically excluded alterations that resolved rapidly on discontinuation of ST use.
Finally, although serum S100B measurements are a valid measure, we cannot entirely exclude alterations of blood brain barrier functioning in alcohol-addicted subjects [ 42] as the cause of these slightly higher initial S100B serum levels.
No significant differences were instead observed in the expression of HER2 and Ki67 (excluding some alteration only evident at the tumour periphery) nor in the average mitotic index in the viable part of the tumours.
These findings exclude preformed alterations in rCBF related to the Vasp−/− genotype and prove that MCA occlusion and reperfusion were sufficient in our model.
Thus, we infer that increased expression levels of VEGF do not underlay the earlier angiogenic switching, although we cannot exclude possible alterations in VEGF bioavailability, mediated for example by increased matrix degrading proteolytic activity.
Nevertheless, these techniques could not exclude indirect alterations of protein expression.
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