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Qiagen RNeasy Plant Mini kit utilises the silica-based membrane column to bind total RNA while RNAzol RT is a single-step total RNA extraction method which excludes the addition of chloroform to phase out the pure total RNA and the separation is done based on the interaction of phenol and guanidine with other cellular components [ 23].
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And vehicles remained fairly affordable last month, having barely risen in price over the last four years, excluding the addition of new options, even as incomes have climbed swiftly in recent years for the affluent families who buy most of the new cars and light trucks these days.
A reference polymer was prepared and coated in exactly the same manner excluding the addition of copper ions.
We agree with the reviewer, 'we cannot exclude the addition of Ca2+ channels to the plasma membrane during the massive vesicular fusion caused by increased hydrostatic pressure.
We cannot exclude the addition of Ca2+ channels to the plasma membrane during the massive vesicular fusion caused by increased hydrostatic pressure.
When the single trial that included participants with CIS alone was excluded, the addition of chemotherapy to BCG was found to reduce both recurrence (relative risk 0.75; 95 % CI 0.61 - 0.92) and progression (relative risk 0.45; 95 % CI 0.25-0.81), with no apparent increase in treatment toxicity [ 13].
A hemichannel-dependent release and, hence, paracrine effect of proapoptotic signals could be excluded since the addition of a peptide (Pep -blocking Cx43-dePep -blockinghannels (but not GJs) did not reduce apoptosis in HeLa-Cx43 cells.
The ICU stay model incorporated all of the above terms with the exception of age and sex, which were excluded, and with the addition of anaesthetist, which was found to be significant in predicting ICU stay.
Nuclei were isolated as described above, excluding formaldehyde crosslinking and the addition of sonic buffer.
To exclude the possibility that the addition of platelets altered the respective protein expression levels in MG63 cells, we performed western blot analysis.
Participants were excluded from the activity analysis if the addition of reported daily physical activity and sedentary behaviour exceeded 16 h, resulting in seven data sets being excluded.
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