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We detected a significant but not complete reduction in FL activity in the PbFL103464(−775 RLef1α parasites compared to the PbFL103464RLef1α parasites, suggesting that both the excluded sequence −989/−775 and the −775/+1 region contain functional portions of the promoter.
These excluded sequence are also available from the BCM-HGSC ftp site.
The sampling step selects a new subsequence from the excluded sequence using a weighting strategy based on the PWM scores.
If the excluded sequence did not score higher than every sequence from the negative dataset it was removed from the final training set.
Bioinformatic analysis revealed that the excluded sequence encodes as many as 6 putative CDK phosphorylation sites and 2 cyclin interaction sites (CY motifs).
Preliminary findings had suggested a link between MLC1 and catatonia via a putative dominantly acting missense mutation cosegregating in a large pedigree, but further analyses have excluded sequence variants of MLC1 as causing chromosome 22q-linked catatonia.
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The excluded sequences are referred to here as redundant sequences.
We expect that a great majority of excluded sequences represent unfiltered PCR artefacts and sequencing errors.
We also excluded sequences composed of >50% gaps as compared to the human sequence.
Second, the excluded sequences are added on the P-core set (the procedure (b) in Methods).
We excluded sequences with translational problems (i.e., frameshift or stop codons).
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