For RNA-seq, another shortcoming is that many short reads likely align to identical reference sequences, which may be excluded from transcript counting, thereby affecting the accuracy of estimated gene expression level [ 42].
To test for the presence of venom-encoding transcripts expressed at high levels but not detectable proteomically, we first excluded from consideration transcripts with anomalous coverage distributions or high discrepancies between transcript estimates as well as all Bradykinin-potentiation and C-type natriuretic peptides for the reasons described above.
The genuine deliberations would take place seven days before the policy is announced and be excluded from full transcripts to be released after eight years.
In some genes, certain exons can be optionally included or excluded from a transcript to produce different versions of the same protein that can often have very different functions.
Consequently, these transcripts designated as putative protein coding could be excluded from the noncoding transcripts with a high reliability.
In one example, the Camk2b gene contains two alternative exons that are mostly excluded from embryonic Camk2b transcripts, but are included in the adult isoform (Brocke et al., 1995).
Convergent overlapping transcripts were excluded from the quantification of antisense transcript levels to avoid potential confusion between sense and antisense transcripts in these cases.
For the exon skipping simulation, one or more exons were excluded from the final transcript for each gene that had at least 3 exons.
Sequencing of the gnz mutant amplicon revealed that both exons 23 and 24 were excluded from the Pign transcript (Fig. 1J).
H9 and BG01V hESCs both express a FGFR1 mRNA including exon 3 (upper band), while all three astrocytic-like cells express a FGFR1 alternatively spliced variant in which exon 3 has been excluded from the mature transcript (lower band).
