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To compare neurons that were activated during the NE (FOS+) to an inactive baseline population, nuclei stained as FOS− but exhibiting high expression of Arc mRNA (TPM > 2.5) were excluded from downstream analysis (11 CA1, 0 DG, and 0 VIP).
Spots flagged as 'Not Found' by the GenePix algorithms were excluded from downstream analysis.
Data were exported to Acuity 4.1, with spots 'Not Found' by the GenePix algorithms excluded from downstream analysis.
Occasionally, replicates gained significant turbidity, suggesting aerobic contamination of the hypoxic environment, and were excluded from downstream analysis.
Such clusters need to be excluded from downstream analysis.
These known germline SNPs were excluded from downstream analysis.
Similar(41)
These loci were excluded from downstream analyses.
Duplicate reads were marked using Picard (http://picard.sourceforge.net/) and were excluded from downstream analyses.
Samples tested positive for genomic DNA were excluded from downstream protocols.
Samples with unusual profiles can be flagged, reprocessed or excluded from downstream analyses (55).
Spots with low signal detectability should be flagged as bad spots and excluded from downstream analyses.
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