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We identified 301 SNPs located at least 100 bp apart between the 2 genomes (we excluded closely located SNPs), among which, 102 were called as positive by Nimblegen algorithm, detecting only 33.9% of the SNPs.
The results of disease-free survival, where the 11 cases of contralateral breast cancer as first event were excluded, closely mirrored those of EFS analyses (data not shown).
In order to efficiently search for sets of significant SNP, we preliminarily pruned SNP, and excluded closely linked SNP having r2 > 0.95 in sliding 50 SNP windows using PLINK [ 26].
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9 This latex agglutination assay could be valuable in correctly identifying select agents and excluding closely related Burkholderia species that cause similar disease in humans.
Yang et al. [ 8] suggested excluding closely related individuals from the analysis when estimating genetic variation captured by all the SNPs, using a GRM-cutoff option.
For example, in medical genetics it is used for excluding closely related individuals from association studies and thereby to avoid inflated false positive rates.
A 10-cM window was used to exclude closely linked cofactors, and the number of control markers was set at five (the default program setting).
This method excludes closely related terminal taxa because the Yule tree prior does not include a model of coalescence, which can complicate rate estimation for closely related sequences [ 69].
This method excludes closely related terminal taxa because the Yule tree prior (see below) does not include a model of coalescence, which can complicate rate estimation for closely related sequences [ 113].
This effect can be reduced using a general linear model (GLM) to estimate the proportion of genes identical by descent between any pair of individuals and excluding closely related individuals [ 18, 19].
For this reason Yang et al. [ 8] suggested excluding closely related individuals from the analysis when estimating heritability from population-based samples, noting that the environmental effect shared by family members seems to be inversely related to their degree of physical proximity, so that close relatives inflate any estimate of heritability.
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