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We excluded called variants in intronic regions beyond 10 bp within the donor and acceptor splice sites, respectively.
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Francis signaled early on that he wanted the church to re-examine its ministry to those who feel excluded, calling bishops to two synod meetings on the family, in 2014 and 2015.
We excluded calls produced by individuals interacting with more than one type of food or when caller identity was uncertain.
From this set of SNPs, we excluded calls whose allele frequency is not between 0.3-0.7 0.3-0.7
CNV calls were created using Birdseye from Birdsuite (version 1.5.5) (50) for autosomes only, and we excluded calls where lengths were <100 kb or >10 Mb, or LOD score <10.
If we excluded calls from single probes and mixed probe calls having a probe vote ratio <75% (for example, one probe suggested a SFP, but three others suggested no SFP), we would have had only four calls that were incorrect (7.7% of the 52).
In addition, we applied a coverage filter at the genotype level to exclude calls with coverage below 2 or above 250.
To facilitate comparison with published findings we did not exclude calls within the telomeric band (but loci containing these calls are flagged where appropriate in subsequent sections of the results).
We then obtained call rates after removing probes with GC content < 16% and > 50% and excluding calls with single probes and multiple probe calls with less than 75% probe votes (Probe filtered).
Correct call rates increased slightly after removing probes with high and low GC contents and increased further after excluding calls from single probes and calls with probe vote ratio < 75%.
A sensitivity analysis for excluding calls with unrecorded age and gender data demonstrated that the exclusion did not significantly impact the EMS demand for all ages and all causes (two-sided p-value of 0.99).
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CEO of Professional Science Editing for Scientists @ prosciediting.com