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Dead cells were excluded by PI staining.
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Cell surface expression of CXCR4 was analysed by staining with an APC-conjugated anti-human CD184 (1 : 50) (12G5) antibody (Pharmingen, Becton-Dickinson, San Jose, CA, USA), and dead cells were excluded by DAPI staining.
A threshold of autofluorescent cells was set with non-transfected cells and the cells expressing C5V were collected on the basis of their fluorescence intensities ranging from 10 to 10. Dead cells were excluded by DAPI staining.
Apoptotic cells were excluded from the analysis by TUNEL staining.
Dead cells were excluded by 7AAD (SIGMA) staining.
(A) Tumor specimens were analyzed by IHC staining for PD-L1, using DAB Peroxidase (HRP) staining technique.
Apoptosis induction was determined by DAPI staining.
Infarct size was measured by TTC staining.
(E) Representative images of apoptotic cardiomyocytes by TUNEL staining.
(D) Representative images of apoptotic cardiomyocytes by TUNEL staining.
Dead cell exclusion was done by DAPI staining.
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