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Here, we report a quantitative analysis of the ability of BAR to exclude the dye bromophenol blue from penetrating into matrix tablets and also sections of hard capsule shells.
Live cells possess intact cell membranes that exclude the dye, whereas dead cells do not.
Cells failing to exclude the dye were considered nonviable.
Viable cells exclude the dye.
Cells failing to exclude the dye were defined as dead cells.
Dead cells could not exclude the dye and showed blue staining of the cell.
Similar(44)
On the contrary, GNP-NKCT1-treated cells showed more intense red fluorescence and reduced green fluorescence since apoptotic and necrotic cells could not exclude the dyes and gave a combination of orange red fluorescence.
After 10 min, a light microscope was used to count and distinguish between non-viable Ascaris eggs that had accumulated the stain (blue), whereas viable eggs had excluded the dye and were colourless.
Live cells excluded the dye, whereas dead cells admitted the dye and consequently stained intensely with trypan blue.
To evaluate viability, cells were mixed with the same volume of 0.4% trypan blue solution, and immediately examined under light microscopy to determine whether they excluded the dye.
Cells excluding the dye were counted using Neubauer cell chamber.
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