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For measurements longer than 4 h after the onset of cell fusion, cycloheximide (CHX) was added to cell cultures to inhibit protein synthesis and thus exclude incorporation of newly synthesized proteins into the mitochondria.
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The lack of Ni signal in EDS spectra does not exclude Ni incorporation within the InN rods, but conclusively confirms its absence at the rod tip, at the typical VLS catalyst placement, commonly observed in VLS growth.
It was expected that proofreading activity at the site of point mutation would exclude the incorporation of a mismatched nucleotide during the gap filling and, thus, improve the specificity of gap-LCR applications.
Although our calculations exclude explicit incorporation of these effects, they indicate that managing Baltic cod fisheries for sustainable yields will require an integrated approach which incorporates ecosystem components such as predator-prey interactions, climate-hydrographic forcing and eutrophication [13], [18], [22], [27], [69].
To exclude Uaa incorporation at legitimate termination sites specified by UAG, endogenous UAG codons in the E. coli genome can be replaced with a synonymous UAA stop codon through genome engineering.
Analyses of the archaeal ~60 bp nucleosomal DNA fragments revealed that A/T-rich oligonucleotides were also excluded from incorporation into archaeal nucleosomes assembled in M. thermautotrophicus and T. kodakarensis.
Heterochromatin has been shown to limit spreading of protein domains in S. pombe (Partridge et al. 2000) and to specifically exclude CENP-A incorporation in Drosophila (Heun et al. 2006).
We could not exclude the possibility of incorporation bias.
Dye-swap experiments were included to exclude effects of disproportionate incorporation of Cy3- or Cy5-dUTP.
Although it is feasible to measure post-radiation H-thymidine incorporation capacity and to exclude patients from protocols whose tumors demonstrate incorporation after gamma-irradiation as was done for the Mel37 clinical trial, we wanted to develop a protocol that would more reliably block H-thymidine incorporation in the irradiated tumor cells.
Single cell clones were then selected to exclude the heterogeneity due to differential incorporation of exogenous genes.
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