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Fluorescence was excited with an excitation filter at 575 605 nm and images were captured at the 645 850 nm range in 10 nm steps with an emission filter 645LP.
Green traces show example evens detected in the channel excited with 470 ± 40 nm light to excite Fluo-4 AM and the red traces show the corresponding data collected with a 548 ± 10 nm excitation filter to excite Syb2-mOrange.
An initial fluorescent measurement was taken using the excitation filter A340/10 to excite at 340 nm and emission was monitored at 505 nm using the F535/25 filter.
Fluo-4 was excited using a 488 nm excitation filter, and detected at 520 nm emission.
Calcein fluorescence was excited and detected using a U-MGFHQ filter cube (Olympus) incorporating a BP460-480 excitation filter, a DM485HQ dichromatic mirror and a BA495-540HQ barriemissionsion) filter.
The uniformed illumination beam passes through the short pass excitation filter (λexccut-off = 500nm), gets reflected by the dichroic filter and excites the sample fluorophores through the microscope objective.
Accent components result from the response to a similar filter, excited with a step function called accent command.
The non-Gaussian process is generated from a non-linear filter excited by a white noise process.
The idea is then to perform the DFT-RDM embedding in the host signal obtained as the output of a whitening filter excited by the colored host.
Phrase components are calculated as the response to a critically damped second order linear filter excited with a delta function called phrase command.
Hence, given a zero-mean white Gaussian host with psd, the colored host can be regarded to as the output of an all-pole filter excited by.
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CEO of Professional Science Editing for Scientists @ prosciediting.com