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An internal clock regulates inflammation in mouse lungs.

Lungs were excised from mice, digested with 20 μg/ml Liberase TL 21 and 50 μg/ml DNase 1 (Sigma) and homogenized using gentleMACS (Miltenyi), as per the manufacturer's instructions.

Blood, bronchoalveolar lavage fluid spleen and lung tissue were excised from mice for analysis.

Peripheral lung tissue was excised from mice and stabilized in RNALater (Qiagen, Hilden, Germany) (n = 6 litters per treatment group, per strain).

For ex vivo biofluorescence imaging (ex vivo BFI), mice lungs were excised after in vivo BLI and placed in the chamber of the NightOwl LB 983 Molecular Light Imager and imaged.

Once excised, the left lungs from sham and PM1 treated mice were fixed in Bouin's solution, embedded in paraffin, cross-sectioned at 7  μm thickness by a rotary microtome, mounted on slides, and stained by hematoxilin and eosin (HE).

H&E stained spleen excised from mice following 14 days treatment after seven intraperitoneal injections.

Four days after CTL transfer, we excised tumor-bearing lungs and made single cell suspensions.

Livers were excised from mice at 44 h after infection.

BAL was collected from the animals before excising the lungs from the chest cavity.

Subsequently, the trachea was exposed and the lungs were excised from the mice by opening the chest via a median sternotomy.