Induction chemotherapy alone or in combination with moderate dose radiotherapy (40 45 Gy), prior to complex surgical excision, may be indicated in selected patients with stage IIIA or IIIB cancers (i.e., those with potentially excisable tumours, and a good performance status) (option).
The negative-selection gene P450 SU1 was incorporated into the excisable cassette as a marker for effective excision.
The cassette was excisable with the Cre-loxP system making possible the removal of the integrated transgenes from the genome.
have previously developed a selectable and excisable marker (SEM) system for the rapid generation of recombinant vaccinia virus.
Recently, human lung disease-specific iPSC lines associated with either cystic fibrosis or alpha-1 antitrypsin deficiency-related emphysema have been generated with a single excisable lentiviral vector[64].
The excisable trait of the LAC12 marker allows the introduction of many different heterologous genes, and makes it possible to introduce a complete heterologous metabolic pathway.
This approach permits direct λRed-mediated cloning of DNA fragment with lengths of 10 kb or greater from the E. coli chromosome into the plasmid vector pGL2, which carries the ori of pSC101, the ϕ80-attP site of ϕ80 phage, and an excisable CmR marker bracketed by λ-attL/attR sites.
We took advantage of the properties of broad-host-range mini-Tn5 vectors and two regulated expression systems (LacIQ/Ptrc and XylS/Pm), together with FRT-flanked, excisable antibiotic resistance determinants, to generate a set of vectors for the delivery of gene(s) into the chromosome of Gram-negative bacteria.
The therapeutic implications of such residual cells have been lessened by the existence of several recently-developed reprogramming techniques that utilize excisable or non-integrating viral vectors that may further improve the safety of these cells for human transplantation.
b, excisable allele; B, non-excisable allele; SLE, systemic lupus erythematosus; VDR, vitamin D receptor.
