Sentence examples for exchange purification from inspiring English sources

For ion exchange purification of the SpeB generated CXCL9 fragments 200 µg of SpeB was incubated with 40 pmol SpeB and 10 mM DTT in 400 µl of PBS.

Samples were loaded on a Zorbax SB-C18 5 µm, 35×0.5 mm (Agilent) trap column and washed for 60 min using 96.7% water: 3% acetonitrile: 0.3% formic acid; the extended wash was to remove residual KCl remaining from the ion exchange purification step.

Ion exchange purification relies on the charge interactions between the sample and the column.

The two differentially labeled reactions to be compared were mixed and buffer exchange, purification, and concentration was accomplished by microcon-10 (Amicon) filtration.

PCR reactions using iPLEX consumables, inactivation by shrimp alkaline phosphatase, extended PCRs, ion exchange purification and spotting onto each SpectroChip II matrix were performed according to the manufacturer's protocols.

As a result of recloning cgAUS1 proenzyme into pGEX-6P-1 the recently developed method could be advanced to successfully produce large amounts of pure, active and soluble cgAUS1 wild type (58 kDa + 26 kDa GST tag) in E. coli and simplify the purification process by a reduction of the four ion exchange purification steps to only one affinity chromatography step [5].

For HPLC analysis of DMA, the supernatants were subjected to ion-exchange purification, evaporated, and filtered under different conditions as previously described (Suzuki et al. 2006).

For HPLC analysis of NA, the supernatants were subjected to ion-exchange purification, evaporated, and filtered as previously described (Wada et al. 2006).

Though expensive, the water could be purified of the radioactive isotopes through ion-exchange purification.

(A ) Anion-exchange purification of M. musculus MAD2R133A.

For the anion-exchange purification step, the protein samples were diluted in a low salt buffer, containing 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.1 mM PMSF, 2 mM β-mercaptoethanol, decreasing the final buffer salt concentration by approximately 5-fold.