Sentence examples for exchange of the medium from inspiring English sources
After exchange of the medium, the cells were incubated for 60 hours.
The exchange of the medium to the one with the original Ca2+ concentration induced constitutive calcium entry in nontransfected cells as well as in double transfectants (YFP-STIM1/ORAI1 and YFP-STIM2/ORAI1) (Figure 6A and C), after which cytoplasmic free Ca2+ levels returned to resting levels characteristic of cells expressing particular proteins (Figure 6A).
All cultures were fed every 2 4 days by complete exchange of the medium.
In this study, cells were grown in a standard submersed cell culture system and then exposed to NPs by exchange of the medium with NP-containing medium.
Induction of apoptosis in bladder cancer cell lines by HA-1077 with or without LPA and GGOH was evaluated after incubation of 1 × 10 cells for 24 hours in serum-free medium, followed by exchange of the medium for 10% FBS containing HA-1077 at its IC50 concentration.
Here we describe the physics and design of the CEA microfluidic device, and demonstrate complete separation of microparticles (polystyrene beads of 4 and 10 μm in diameter) and efficient exchange of the carrier medium while retaining 10 μm beads.
The requirement of TolC for growth at low pH is surprising because TolC resides in the outer membrane, mediating exchange of the external medium with the periplasm; and the periplasmic pH generally equals the external pH [23].
The DETFs of the TFN can roughly be divided into 2 groups describing the main biological functions observed as a response upon the exchange of the culture medium.
Since the present study uses the data of Zellmer et al. but focuses on the second phase of the experiment (24 hours after the exchange of culture medium), the aftereffects of the proliferation initiation and cell cycle regulation were monitored as well.
On day 3 of culture, additional 10 ml medium was added, exchanging half of the medium on day 6.
Subsequent medium exchanges involved 50% of the medium being removed and replaced but without nanoparticles.
