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The modified anion exchange media, core-shell adsorbents (InertShell and InertLayer 1000) and polymer grafted adsorbents (SQ) were compared.
Consequently, the selective adsorption and desorption of acidic molecules is built into our extraction method, and replaces the equally effective subsequent capture on anion exchange media.
The current study was carried out using strong anion exchange media packed in a cuboid packed-bed device and its equivalent column, i.e. with identical volume and bed-height.
Models have been developed to simulate the thermal characteristics of crystalline silicotitanate ion exchange media fully loaded with radioactive cesium either in a column configuration or distributed within a waste storage tank.
Microscale packed-bed chromatography columns (5 to 80 μL) were designed with cylindrical polyetheretherketone (PEEK) tubes for preparative ion exchange media by reducing internal column diameters with retained heights.
The selective cesium capacity of this inorganic ion exchange media was evaluated with simulated sodium bearing waste (SBW) and dissolved pilot plant calcine at the Idaho Nuclear Technology and Engineering Center INTECC).
Similar(46)
Varying the ligand ratio (SP/Phenyl) could be used for optimizing the function of mixed-ligand ion-exchange media.
However, for phenol derived anion-exchange media the recovery can be improved by desorption at high pH.
Overall, the information provided in this paper aims at the eventual development of rational design or selection strategies of ion-exchange media for the satisfactory and successful refolding of a target protein.
It was contrived with the purposes of (i) providing sufficient osmolarity and buffering capacity, (ii) minimizing ionic species to facilitate the binding of extracellular proteins to anion-exchange media, and (iii) enhancing PAC expression level and secretion efficiency.
The application of ion-exchange (IEX) chromatography to protein refolding (IExR) has been successfully proven, as supported by various studies using different model proteins, ion-exchange media and flow configurations.
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