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This observation indicates that NHEJ or non-allelic HR (NAHR) are potential sources of chromatid exchange formation.
Therefore, we propose that DSB misrejoining by NHEJ is the main cause of chromatid exchange formation in hESCs and differentiated cells.
Chromosomal aberra-tions observed in the G2 assay are formed within 2 hours after irradiation, and thus, fast repair is more likely to play a role in chromatid exchange formation.
To determine the mechanisms of chromatid exchange formation in our system, we analyzed the dose-response relationship of chromatid exchanges induced by irradiation at G2 at 0.25, 0.75, and 1 Gy for hESM01, hESM01f and HS27 cell lines.
The rate of radiation-induced chromatid exchanges in hESCs and differentiated cells exhibited a quadratic dose response, revealing two-hit mechanism of exchange formation suggesting that a non-homologous end joining (NHEJ) repair may contribute to their formation.
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"Following the principles of initiative, controllability and gradualism, with reference to international capital flows and changes in major currencies, we will improve the yuan exchange rate formation mechanism," the central bank said in a 46-page monetary policy report.
Further ionic exchange toward formation of Li4VO(PO4)2 lowers the symmetry.
Additions of perchloric acid can promote a ligand exchange with formation of mixed chloro-complexes.
Finally, solvent exchange, alcogel formation and scCO2 drying allowed the manufacture of the desired nanometre-scale fibrous PLA aerogels.
In-cylinder flow simulations including gas exchange, mixture formation, combustion, and knock were carried out and the results are compared with experimental data.
By combination of electrochemical methods with X-ray photoelectron and Raman spectroscopy it was shown that the redox transformations observed result in ligand exchange and formation of nickel hydroxide thin films deposited on the working electrodes.
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