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Purification of the non-tagged TM0415 for crystallization was performed by heat treatment, anion exchange, and size exclusion chromatography.
The His-tagged TM0415 enzymes for kinetic analysis were purified by Ni affinity, anion exchange, and size exclusion chromatography.
Purification methods are currently dominated by ion exchange and size exclusion chromatography.
Through a combination of ion exchange and size exclusion chromatography, we enriched a single active protein, the lactate dehydrogenase isozyme LDHC, which is primarily expressed in testis.
From this culture, a laccase was purified through diafiltration and anion exchange and size exclusion chromatography, with a purification factor of 7.45 and a 0.51% yield.
PP80 exhibited a high proton mass transfer coefficient (KH = 11 × 10−5cm/s) compared to all ion exchange and size selective separators included in this study.
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Briefly, purified fractions of caudal sperm extracts produced by anion exchange and size-exclusion chromatography were preincubated in a buffer containing 1 mM Mn2+ in a total volume of 30 µl at 30°C for 5 min, followed by addition of labeled phosphorylase a.
Protein purification was carried out using sequential NiNTA affinity, anion exchange, and size-exclusion chromatographies.
Polysaccharides from the leaves of Clerodendrum splendens were extracted and fractionated by ion exchange and size-exclusion chromatography.
Mboumar-9 transposase, containing the same mutation (T216A) that rendered the recombinant Mos1 protein soluble, was expressed in E. coli and purified by cation exchange and size-exclusion chromatography (panels a and b of Figure 2, respectively), as described in Materials and Methods.
The crosslinking reaction was terminated with excess DTT and the products were purified using ion exchange and sizing chromatography.
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