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Drained excessive solution in <10 s.
Emerged the rack in Fixer for 4 min and then drained excessive solution.
Excessive solution was removed by an absorbent paper, and the sample was dried at room temperature in air.
After the excessive solution was removed, the treated slides with the mites were kept at 25°C (± 2°C) in a Petri dish with moist filter paper.
Drained excessive solution in <10 s. (C) Washed the rack in water for 1 min and drained excessive water as much as possible.
GA has shown to be efficient on global optimization by finding the most promising regions of search space; however, it suffers from excessive solution time and low accuracy.
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After staining with a Live/Dead assay and washing with DPBS to remove excessive staining solution, the stained and unstained cells were mixed to prepare 10% to 50% volume fraction heterogeneous solutions.
To remove excessive staining solution, cells were washed 3 times with tab water.
After 45 min on ice, excessive enzyme solution was replaced by 5 - 20 μl of buffer without enzyme, and proteins were digested at 37°C overnight.
After 6 days, tissues were gently washed in ultrapure water (3 × 1 min), tissues were gently blotted to remove excessive dichromate solution, and placed in aqueous 0.75 % silver nitrate for 5 days in the dark (50 1 fluid to tissue volume ratio).
A generalization of TVD concepts is employed to design solution-dependent antidiffusive fluxes which are inserted into the defect vector to preclude excessive smearing of solution profiles by numerical diffusion.
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