Sentence examples for excess synthetic from inspiring English sources

However, Ca2+- calmodulin can be displaced by excess synthetic peptides, derived either from the competitive IP3R motif or from the heterologous myosin light chain kinase.

Controls using preimmune serum and antiserum absorbed with excess synthetic peptide or omission of primary or secondary antibody revealed no labeling.

Excess synthetic peptide (MW 3 kDa) was removed by extensive dialysis (1 400 vol:vol) over 24 48 hours with at least 3 buffer exchanges using 12 kDa MW cutoff pre- moistened dialysis membrane (Slidealyzer), as well as during concentration with a 10 kDa MW cutoff concentrator to >5 mg/ml (>100 μM) semisynthetic PTEN.

In the present study, we propose that exposure to excess prenatal synthetic glucocorticoids results in maternal adaptations to pregnancy, altered epigenetic regulation of gene expression, or a combination of both.

Such glasses were prepared by Sinclair et al. by blending biogenic carbonate (coral powder) with silica in a ratio of 1 1, followed by fusion at 1650 °C and by Perkins et al. by blending Li2B4O7 in excess with synthetic CaCO3, MgO, and gravimetric additions of the analytes of interest, followed by fusion over a burner flame [ 26, 27].

Brain sections were treated with anti-HCRTR-1, previously incubated (for 12 24 h at 4 °C, with mild agitation) with the specific peptide it recognizes (3- to 5-fold molar excess of synthetic peptide, relative to IgG concentration at the normal antibody dilution).

Release of myc-containing protein complexes from beads was carried out by exposing the beads to an excess of the synthetic epitope-mimicking myc peptide described above.

In controls the ERβ2 antibody was preincubated with a molar excess of immunising synthetic peptide (CMKMETLLPEATMEQ [ 18]) prior to application to sections from positively-staining specimens.

Negative controls included preabsorption of the primary antibody with an excess of corresponding synthetic peptide (sc-350 p, 100  μg 0.5−1 ml, Santa Cruz Biotechnology, Inc), and the use of isotype-matched nonimmune rabbit IgG.

We confirmed that the KAP3 signal in LBHIs was not present when the anti-KAP3 antibody was pre-incubated with an excess of the synthetic KAP3 antigen peptides (data not shown).

The human DNA2 α1-RPA70 OBN complex was crystallized by mixing an 8.7 mg/ml solution of the RPA70N polypeptide (residues 1 120) with a 3-fold molar excess of a synthetic DNA2 α1 peptide (residues 1 20) from 50 mM Tris-HCl, 35% PEG 1500, 2 mM TCEP, pH 8.0.